Since 2005, Professor Monique van Hoek of Mason’s School of Systems Biology (SSB), has studied the bacteria Francisella, which can cause tularensis, a serious disease. Francisella, found in animals — especially rodents, rabbits, and hares — is also considered a class “A” biothreat agent. In 2017-2018, van Hoek extended her Francisella study when she was awarded a 4-VA grant “Critical post-translational modifications of the Francisella proteome.” This research brought her together with Dr. Kristina Nelson, Director, Chemical Mass Spectrometry Resource (CMSR) at the Chemical and Proteomic Mass Spectrometry Core Facility at Virginia Commonwealth University. Nelson received 4-VA Complementary Funding to assist in van Hoek’s study and a scientific match was made.
Recognizing the benefits of the SSB and the CMSR collaboration, this relationship has endured. As van Hoek recognized, there is much more to be learned about this unusual bacterium, so in 2019 she applied and received a second 4-VA grant for a continuation of the study to research “Secreted proteins of Francisella.”

The Mason-VCU collaboration resulted in another success story. Together, van Hoek and Nelson performed experiments and proteomics to identify the secreted proteins of Francisella novicida, a model bacterium used to safely study the more dangerous form that causes human infections. Explains van Hoek, “We examined the proteins for signal peptides encoded in the proteome of Francisella and we identified 129 proteins of F. novicida that were predicted to have secretory signal peptides. These secreted proteins will advance our understanding of the pathogenicity of Francisella.”
Joining van Hoek and Nelson in the research was WeiDong Zhou, Research Associate Professor, Center for Applied Proteomics and Molecular Medicine, and volunteer graduate student Fasial Madkhali.
Now, they are sharing the results of their research; first through an abstract accepted for the Virginia Academy of Sciences in 2020. van Hoek is working on a manuscript on the topic and hopes to apply for a NIH grant to do further research.
“This 4-VA@Mason grant allowed us another valuable opportunity to share resources and accomplish much more together as we could have done alone,” van Hoek concludes.



























